Tissue biopsy and imaging studies in hiv-infected patients – full text view – clinicaltrials. gov

This study will examine tissue from the tonsils, lymph nodes and large bowel of HIV-infected patients to investigate changes in viral load and certain white blood cells during treatment.

Normal volunteers and HIV-infected patients 18 years of age or older may be eligible for this study. Endometrial biopsy procedure Candidates will be screened with a medical history, physical examination, blood and urine tests and possibly an electrocardiogram (EKG).

Endometrial biopsy side effects Blood tests may include HLA typing, a genetic test of immune system markers.

• Biopsies The frequency of biopsies for given patients may vary, depending on their specific therapy. Liver biopsy recovery time Typically, biopsies are done at a single time, or for patients starting a new therapy, biopsies could be performed before starting therapy, during therapy and possibly after completion of therapy.

• Tonsil biopsies (patients and volunteers) Volunteers will have one tonsil biopsy. Abnormal endometrial biopsy results Patients will have no more than six tonsil biopsies, with no more than three in a 10-day period. Endometrial biopsy recovery The biopsy is done by an ear, nose and throat specialist as an outpatient procedure. Endometrial biopsy video The tonsils are numbed with a local anesthetic, and one to four pieces of tissue are extracted.

• Lymph node biopsies (patients only) Patients will have no more than four lymph node biopsies, performed no more frequently than once a month. Pain after endometrial biopsy The biopsy is done by a surgeon and may require a 2- to 3-day hospital stay. Cervical biopsy recovery The skin above the lymph nodes is numbed with a local anesthetic, an incision is made and the tissue is removed. Endometrial biopsy complications Alternatively, a needle biopsy may be done, in which a small amount of lymph tissue is withdrawn through a special needle injected into the site.

• Intestinal biopsies (patients and volunteers) Volunteers will have one intestinal biopsy procedure. Hysteroscopy endometrial biopsy Patients may have up to six intestinal biopsy procedures, each separated by at least 10 days. Breast biopsy recovery time This is done by a gastroenterologist as an outpatient procedure. Endometrial biopsy ivf A flexible tube (sigmoidoscope or colonoscope) with a light and special lens at the tip is inserted into the rectum and large bowel. Endometrial biopsy pain Wire instruments passed through the tube are used to extract small tissue samples.

• Bronchoalveolar lavage (BAL; patients and volunteers) Volunteers and patients will undergo bronchoscopy in which a flexible tube (bronchoscope) with a light and special lens at the tip is inserted through the nose or mouth into the lungs, and the lining of the lung is sampled by washing the airways with small amounts of saline. Breast biopsy recovery The procedure is performed by a pulmonologist or critical care specialist, usually as an outpatient.

Examining tissues outside the bloodstream in HIV-infected patients, patients with Idiopathic CD4 lymphopenia (ICL) and, for comparison, HIV-uninfected healthy volunteers (hereafter referred to as healthy volunteers), can provide insights into the pathogenesis of HIV infection and ICL. Endometrial biopsy results time frame This protocol will provide a mechanism for sampling tissue sites. Endometrial biopsy results interpretation A total of 430 HIV-infected patients, 100 ICL patients and 105 healthy volunteers will be enrolled in these studies. Endometrial biopsy results To assess changes in viral load and immunological parameters at sites outside the bloodstream during therapy of HIV-infected patients, sequential tonsillar, lymph node, or intestinal biopsies, or bronchoscopy with bronchoalveolar lavage (BAL) will be undertaken during the course of therapy. Bleeding after endometrial biopsy In a small number of patients, sequential or simultaneous lymph node biopsies will be performed. What is endometrial biopsy In addition, uninfected healthy volunteers will be enrolled to have a tonsillar or intestinal biopsy, or bronchoscopy with BAL; this will allow comparison of immunologic parameters in HIVinfected and uninfected tonsillar or intestinal tissues, or BAL fluid. Endometrial biopsy results how long Finally, ICL patients may have tissue sampling to assess lymphocyte distribution and possible function in tissues to better understand the pathogenesis of their lymphopenia. What is an endometrial biopsy Sequential or simultaneous tissue sampling may occur and longitudinal samples may also be obtained to assess stability in tissue compartments or effect of possible immunomodulatory treatments.

Willingness to allow storage of blood or biopsy samples for possible future use to study HIV/AIDS, related diseases or the immune system; willingness to permit HLA testing.

No aspirin or piroxicam (Feldene) for 10 days prior to the procedure; other non steroidal anti-inflammatory drugs (e.g. Biopsy endometrial ibuprofen) must be discontinued the day prior to the procedure. Skin biopsy results time Acetaminophen [Tylenol] is permitted at any time.

HIV infection must be confirmed by ELISA and western blot or dot blot. Pipelle endometrial biopsy For patients with acute HIV infection and negative HIV serology, plasma HIV viral load greater than 10,000 copies/ml.

Patients must meet the definition of ICL according to the CDC criteria: documented absolute CD4 T lymphocyte count of less than 300 cells per cubic millimeter or of less than 20 percent of total T cells on more than one occasion usually two to three months apart, without evidence of HIV infection or any defined immunodeficiency or therapy associated with depressed levels of CD4 T cells.